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Mice susceptible to SARS coronavirus
David E Wentworth1, Laura Gillim-Ross, Noel Espina
1New York Department of Health, Albany 12201-2002, USA. dwentwor@wadsworth.org
Emerging Infectious Diseases
|August 25, 2004
Summary
Developing a murine model for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is crucial. This study found SARS-CoV replicated in both lung and intestinal tissues of mice after intranasal and oral inoculation.
Area of Science:
- Virology
- Infectious Diseases
- Animal Models
Background:
- Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is an emerging viral threat.
- Effective research models are needed to study SARS-CoV pathogenesis and develop countermeasures.
Purpose of the Study:
- To establish and characterize a murine model for SARS-CoV infection.
- To investigate the tissue tropism of SARS-CoV in a small animal model.
Main Methods:
- BALB/c mice were inoculated intranasally and orally with SARS-CoV.
- Viral replication was assessed in lung and intestinal tissues.
Main Results:
- SARS-CoV replicated successfully in the established murine model.
- Viral replication was detected in both the lung and intestinal tissues of inoculated mice.
Conclusions:
- The intranasal and oral inoculation route effectively establishes SARS-CoV replication in BALB/c mice.
- This murine model provides a valuable tool for studying SARS-CoV infection, particularly its effects on the respiratory and gastrointestinal tracts.