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Related Experiment Videos

URB expression in human bone marrow stromal cells and during mouse development.

Yi Liu1, Massimiliano Monticone, Laura Tonachini

  • 1Dipartimento di Oncologia, Biologia e Genetica, Università di Genova, 16132 Genova, Italy. yiliu@cba.unige.it

Biochemical and Biophysical Research Communications
|August 25, 2004
PubMed
Summary

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The URB gene is highly expressed in undifferentiated bone marrow stromal cells (BMSC) but downregulated during osteoblast differentiation. Its expression pattern in mouse development suggests a role in skeletogenesis.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Seven genes were previously identified with preferential expression in undifferentiated human bone marrow stromal cells (BMSC) compared to BMSC-derived osteoblasts.
  • The URB gene, belonging to the sushi-repeat-containing protein superfamily, was among these identified genes.

Purpose of the Study:

  • To characterize the expression of the URB gene in human and mouse models.
  • To investigate the role of URB during osteoblastic differentiation and embryonic development.

Main Methods:

  • Differential display
  • Cell culture of human BMSC and osteoblasts
  • Analysis of URB expression in mouse tissues and embryos
  • In situ hybridization

Related Experiment Videos

  • Immunohistochemistry

    • Main Results:

    • URB is expressed in human primary and cloned BMSC and significantly downregulated during osteoblastic differentiation.
    • A single 3.8kb Urb transcript is detected in mouse tissues.
    • Mouse Urb protein is secreted as a 150kDa glycoprotein.
    • Urb RNA is minimally detected in early mouse embryos (9dpc) and increases in later stages.
    • Urb expression is primarily observed in cartilage during mouse embryonic development (from 14dpc).

    Conclusions:

    • The URB gene is downregulated during osteoblastic differentiation in human cells.
    • The temporal and spatial expression pattern of URB in mouse embryos suggests a role in skeletogenesis.