Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Introduction to protein crystallization.

Alexander McPherson1

  • 1Department of Molecular Biology and Biochemistry, University of Califonia, Irvine 560 Steinhaus Hall, Irvine, CA 92697-3900, USA. amcphers@uci.edu

Methods (San Diego, Calif.)
|August 25, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A note on the appearance of PEG in macromolecular crystals.

Acta crystallographica. Section F, Structural biology communications·2025
Same author

The growth of microcrystals for time resolved serial crystallography.

Methods in enzymology·2024
Same author

Structures of additional crystal forms of Satellite tobacco mosaic virus grown from a variety of salts.

Acta crystallographica. Section F, Structural biology communications·2021
Same author

The crystal structure of the β subunit of luteinizing hormone and a model for the intact hormone.

Current research in structural biology·2021
Same author

Structures of two novel crystal forms of Aspergillus oryzae alpha amylase (taka-amylase).

Journal of bioscience and bioengineering·2021
Same author

Crystal structure of a proteolytically cleaved, amino terminal domain of apolipoprotein E3.

Biochemical and biophysical research communications·2020
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

This review summarizes techniques for crystallizing biological macromolecules using various reagents and physical conditions. It serves as an introduction to successful crystallization for X-ray structure determination.

Area of Science:

  • Structural Biology
  • Biochemistry

Background:

  • Crystallization is crucial for determining the 3D structure of biological macromolecules.
  • Success in macromolecular crystallization often relies on empirical screening of diverse conditions.

Purpose of the Study:

  • To provide a foundational overview of biological macromolecule crystallization techniques.
  • To outline fundamental approaches and influencing factors for successful crystallization.
  • To serve as an introductory guide for researchers, particularly novices, aiming for X-ray structure determination.

Main Methods:

  • Summarizes various crystallization techniques.
  • Discusses the use of diverse reagents to achieve supersaturation.
  • Highlights the application of different physical conditions, such as temperature variations.

Related Experiment Videos

Main Results:

  • Provides a framework for understanding the principles of macromolecular crystallization.
  • Identifies key factors that influence the success of crystallization experiments.
  • Offers a stepping-stone for novice researchers in the field.

Conclusions:

  • Successful crystallization is achievable through systematic exploration of techniques, reagents, and conditions.
  • This review offers a valuable introduction to macromolecular crystallization for X-ray crystallography.
  • Understanding crystallization parameters is key to advancing structural biology research.