[Flocculating mechanism of microbial flocculant MBFTRJ21]

The bioflocculant MBFTRJ21 for nature soda was exploited in our lab, and its flocculating mechanism was studied. The bioflocculant produced by Lactobacillus, has some advantages, such as less dosage, better flocculating effects, and etc. MBFTRJ21 is mainly composed of polysaccharide, which is determined by Anthrone reaction, Coomassie blue reaction and Elson-Morgan method. The acting force between bioflocculant and nature soda is hydrogen bond, which is determined by xi electric potential, hydrogen bond and electrovalent bond determination. The bioflocculant is treated by heat and KIO4, which indicate the active ingredient of bioflocculant is protein and osamine. Bridge occur during flocculation process, which is determined by particle size distribution. The results showed that the flocculating mechanism of MBFTRJ21 resulted from the combination of nature soda with MBFTRJ21 by hydrogen bond at the active position of glycosaminoglycan.