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Updated: Aug 22, 2026
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
Published on: December 3, 2019
Identification of region-specific transcription factor genes in the adult mouse brain by medium-scale real-time
Harukazu Suzuki1, Rie Okunishi, Wataru Hashizume
1Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. rgscerg@gsc.riken.jp
Abstract:
We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.
