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Related Experiment Videos

Thioredoxin can influence gene expression by affecting gyrase activity.

Kuanyu Li1, Cécile Pasternak, Elisabeth Härtig

  • 1Institut für Mikrobiologie und Molekularbiologie, University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.

Nucleic Acids Research
|August 26, 2004
PubMed
Summary

Thioredoxins (TrxA and TrxC) in Rhodobacter bacteria regulate photosynthetic gene expression by interacting with DNA gyrase, altering DNA supercoiling. This novel pathway links oxygen levels to gene control in bacteria.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Genetics

Background:

  • Oxygen tension regulates gene expression in facultative photosynthetic bacteria like Rhodobacter.
  • The puf and puc operons, encoding photosynthetic apparatus proteins, are oxygen-regulated.
  • Thioredoxins were previously implicated in regulating these operons, but the mechanism was unknown.

Purpose of the Study:

  • To elucidate the mechanism by which thioredoxins influence the expression of puf and puc operons in Rhodobacter.
  • To identify the interaction partners of thioredoxins involved in gene regulation.
  • To understand the role of DNA supercoiling in oxygen-regulated gene expression.

Main Methods:

  • Investigated the interaction between thioredoxins (TrxA, TrxC) and DNA gyrase in Rhodobacter capsulatus and Rhodobacter sphaeroides.

Related Experiment Videos

  • Assessed the effect of thioredoxins on DNA gyrase's supercoiling activity.
  • Examined the impact of inhibiting DNA gyrase activity on puf and puc gene expression.

    • Main Results:

    • Reduced TrxA and oxidized TrxC interact with DNA gyrase.
    • TrxA enhances DNA supercoiling activity, while TrxC inhibits it.
    • Inhibition of DNA gyrase activity significantly reduces puf and puc gene expression.

    Conclusions:

    • Thioredoxins modulate bacterial gene expression through interaction with DNA gyrase and regulation of DNA supercoiling.
    • A novel signaling pathway involving thioredoxins, DNA gyrase, and DNA supercoiling mediates oxygen-dependent gene expression in Rhodobacter.
    • This finding provides new insights into the mechanisms controlling gene expression in response to environmental cues.