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Related Experiment Videos

Altered epithelial-basement membrane interactions in diabetic corneas.

D T Azar1, S J Spurr-Michaud, A S Tisdale

  • 1Eye Research Institute, Harvard Medical School, Boston, Mass. 02114.

Archives of Ophthalmology (Chicago, Ill. : 1960)
|April 1, 1992
PubMed
Summary

Diabetes alters corneal basement membranes, reducing hemidesmosome formation sites in both diabetic and normal corneal cells. This suggests impaired cell-extracellular matrix interactions in diabetic corneas.

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Area of Science:

  • Ophthalmology
  • Diabetic Complications
  • Cell Biology

Background:

  • Diabetes mellitus is associated with morphologic changes in the extracellular matrix.
  • These changes include basement membrane thickening and reduced hemidesmosome coverage in corneal epithelium.

Purpose of the Study:

  • To investigate if diabetic basement membranes alter epithelial recognition of hemidesmosome formation sites.
  • To determine if diabetic epithelium can recognize hemidesmosome sites on normal basement membranes.

Main Methods:

  • In vitro recombination of diabetic and control corneal epithelial sheets with stromas and denuded basement membranes.
  • Quantification of the area of the basal cell membrane occupied by hemidesmosomes.

Main Results:

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  • Diabetic epithelium on normal stroma showed significantly reduced hemidesmosome area (10.7%) compared to controls (19.5%).
  • Normal epithelium on diabetic stroma also showed significantly decreased hemidesmosome area (8.6%).

Conclusions:

  • Diabetic corneas exhibit altered cell-extracellular matrix interactions.
  • Both basement membrane abnormalities and epithelial cell changes in diabetes may contribute to reduced hemidesmosome formation.