Optimizing RNA extraction yield from whole blood for microarray gene expression analysis
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Updated: Aug 22, 2026
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
Published on: November 28, 2018
Jian Wang1, John F Robinson, Hafiz M R Khan
1Vascular Biology Research Group and London Regional Genomics Centre, Robarts Research Institute and University of Western Ontario, London, ON, N6A 5K8, Canada.
Objectives:
Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications.
Design And Methods:
We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip.
Conclusions:
Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates.
