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Effect of pre-analytical variables on glycohemoglobin measurements in routine clinical care
Joíza L Camargo1, Mariane Felisberto, Jorge L Gross
1Clinical Pathology Department, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil. jcamargo@hcpa.ufrgs.br
Objectives:
To analyze the contribution of the labile A1C fraction (L-A1C) and sample storage conditions on GHb measurements.
Design And Methods:
L-A1C separation (HPLC Hitachi L-9100) was evaluated before and after sample treatment by four different methods for L-A1C removal. Different sample storage conditions were analyzed.
Results:
L-A1C may overestimate up to 1.64% total A1C if not properly separated. Samples were stable for up to 10, <1, and at least 100 days for whole blood stored at 4, -20, and -80 degrees C, respectively.
Conclusions:
The presence of L-A1C may overestimate GHb results. Samples may be kept under refrigeration up to 10 days and at -80 degrees C if long-term storage is required.
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