Related Experiment Videos
Agarose-coated anion exchanger prevents cell-adsorbent interactions
Maria E Viloria-Cols1, Rajni Hatti-Kaul, Bo Mattiasson
1Department of Biotechnology, Center of Chemistry and Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
Abstract:
A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The cross-linked agarose layer was stable during recycling of the adsorbent.