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Updated: Aug 22, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
Simultaneous operation of plural separation modes in capillary electrophoresis with a chemiluminescence detector
Kazuhiko Tsukagoshi1, Takashi Tokunaga, Riichiro Nakajima
1Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan. ktsukago@mail.doshisha.ac.jp
Abstract:
Analysis of alpha-amino acids, proteins, and phenolic compounds was simultaneously performed using three capillaries in capillary electrophoresis with chemiluminescence detection, taking advantage of the micro-space area for reaction/detection at the tip of the capillary. The three capillaries included usual, polymer-containing, and sodium docley sulfate (SDS)-containing migration buffers for separation. The eluted samples from the capillaries, which were inserted into the chemiluminescence detection cell, were mixed with chemiluminescence reagent at the tips of the capillaries to generate visible light. The specific micro-space area for reaction/detection at the tips of the capillaries enabled the simultaneous operation of the three separation modes in the present system.
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