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Characteristics of core promoter and precore stop codon mutants of hepatitis B virus in Vietnam

Tran Thien Tuan Huy1, Hiroshi Ushijima, Vo Xuan Quang

  • 1Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.

Journal of Medical Virology
|August 28, 2004
PubMed

Insights

Hepatitis B virus (HBV) genotype C in Vietnam is linked to severe liver disease, including cirrhosis and cancer. Core promoter mutations are associated with genotype C and disease severity in chronic HBV infections.

Area of Science:

  • Hepatology
  • Virology
  • Genetics

Background:

  • Hepatitis B virus (HBV) genotypes B and C are prevalent in Asia, with genotype C associated with more severe liver disease.
  • Core promoter/precore (CP/PC) mutants of HBV are linked to specific genotypes and may influence disease progression and severity.

Purpose of the Study:

  • To investigate the prevalence of CP/PC mutants in Vietnamese patients with acute and chronic HBV infections.
  • To determine the association between HBV genotypes, CP/PC mutations, and the severity of liver disease.

Main Methods:

  • Sera from 115 Vietnamese HBV-infected patients (39 acute, 76 chronic) were analyzed.
  • Tests included liver profile, HBeAg, HBV genotypes, and HBV DNA levels.
  • Fragments covering CP/PC regions were amplified, sequenced, and analyzed.

Main Results:

  • In acute HBV, CP/PC mutants were found in 38.4% and 25.6% of cases. Genotype B was predominant (74.3%) and linked to the PC mutant A1896.
  • In chronic HBV, CP/PC mutants were found in 61.7% and 32.8% of cases. The T1762/A1764 double mutant correlated significantly with genotype C (81%), liver cirrhosis, and hepatocellular carcinoma.
  • Genotype C, with a high rate of C-1858 (70%), was strongly associated with severe chronic liver disease.

Conclusions:

  • HBV genotype C, particularly with specific CP/PC mutations like T1762/A1764 and C-1858, plays a significant role in the pathogenesis of severe chronic liver disease in Vietnam.
  • Understanding these genotype-mutant associations is crucial for predicting disease progression and managing HBV infection in endemic regions.

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