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PER-1 production in a urinary isolate of Providencia rettgeri

G Bahar1, B Eraç, A Mert

  • 1SSK Educational Hospital Clinical Microbiology, Ankara, Turkey. gulbahkar@hotmail.com

Journal of Chemotherapy (Florence, Italy)
|August 31, 2004
PubMed

Insights

A Providencia rettgeri strain producing the PER-1 extended-spectrum beta-lactamase was identified. This enzyme confers resistance to cephalosporins and is now described in P. rettgeri for the first time.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Extended-spectrum cephalosporins are crucial antibiotics.
  • Extended-spectrum beta-lactamases (ESBLs) are enzymes that confer resistance to these drugs.
  • The PER-1 enzyme is prevalent in Turkey, particularly in Acinetobacter baumannii and Pseudomonas aeruginosa.

Purpose of the Study:

  • To characterize an antibiotic-resistant Providencia rettgeri isolate.
  • To identify the specific beta-lactamase enzyme responsible for resistance.
  • To report the first instance of PER-1 enzyme in P. rettgeri.

Main Methods:

  • Bacterial isolation and antimicrobial susceptibility testing.
  • Phenotypic detection of extended-spectrum beta-lactamase activity using clavulanic acid.
  • Molecular detection of the PER-1 gene using Polymerase Chain Reaction (PCR).

Main Results:

  • A Providencia rettgeri strain exhibited resistance to extended-spectrum cephalosporins and intermediate resistance to aztreonam.
  • Clavulanic acid restored susceptibility to extended-spectrum cephalosporins, indicating ESBL production.
  • PCR confirmed the presence of the PER-1 gene in the isolated strain.

Conclusions:

  • This study reports the first detection of the PER-1 extended-spectrum beta-lactamase in a Providencia rettgeri isolate.
  • The findings highlight the potential for carbapenemase spread among different bacterial species.
  • This underscores the importance of continuous surveillance for antibiotic resistance mechanisms in clinical settings.

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