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Strain differences in murine ventilatory behavior persist after urethane anesthesia.

Iahn Gonsenhauser1, Christopher G Wilson, Fang Han

  • 1Division of Pulmonary and Critical Care Medicine, Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4941, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|August 31, 2004
PubMed
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Genetic differences in mouse respiratory patterns persist even under anesthesia. A/J mice consistently exhibit slower, deeper, and more variable breathing than B6 mice, indicating robust genetic control of breathing. The neural basis for these traits is partly subcortical.

Area of Science:

  • Neuroscience
  • Respiratory Physiology
  • Genetics

Background:

  • Significant differences exist in breathing patterns between awake C57BL/6J (B6) and A/J mouse strains.
  • A/J mice display slower, deeper, and more variable breathing compared to B6 mice.
  • Urethane anesthesia's impact on cortical and subcortical function was investigated to test if a fully functional neuroaxis is required for these strain differences.

Purpose of the Study:

  • To investigate the hypothesis that strain-specific breathing differences in mice require a fully functional neuroaxis.
  • To determine if urethane anesthesia alters or maintains the distinct respiratory patterns observed between B6 and A/J mouse strains.

Main Methods:

  • B6 and A/J mice were anesthetized with urethane and placed in a whole-body plethysmograph.

Related Experiment Videos

  • Respiratory frequency (Fr), inspiration/expiration durations, and amplitude were measured under normoxia, hyperoxia, hypoxia, and post-hypoxia.
  • Breathing variability was quantified using the coefficient of variation (CV) and spatial statistics on Poincaré plots.

    • Main Results:

    • Anesthetized A/J mice maintained slower, deeper, and more variable breathing than B6 mice at rest and during hyperoxia.
    • During hypoxia, respiratory frequency and its variability were not significantly different between strains.
    • Post-hypoxia, B6 mice showed reduced Fr and increased variability compared to baseline, unlike A/J mice, with strain differences confirmed by spatial analysis.

    Conclusions:

    • Strain-specific differences in respiratory pattern and variability are robust genetic traits in mice.
    • These distinct respiratory characteristics are maintained under urethane anesthesia.
    • The neural substrates underlying these differences are, at least partially, located within subcortical structures.