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Related Experiment Videos

Gender and sodium-ascorbate transporter isoforms determine ascorbate concentrations in mice.

Shiu-Ming Kuo1, Marlene E MacLean, Kathleen McCormick

  • 1Department of Exercise and Nutrition Sciences, University at Buffalo, Buffalo, NY, USA.

The Journal of Nutrition
|August 31, 2004
PubMed
Summary

Sodium-dependent vitamin C transporters SVCT1 and SVCT2 regulate ascorbate levels. SVCT2 is crucial for tissue accumulation, while females exhibit distinct ascorbate handling due to lower excretion.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Physiology

Background:

  • Vitamin C (ascorbate) is essential, and its tissue concentrations are regulated by specific transporters.
  • Sodium-dependent vitamin C transporters (SVCT1 and SVCT2) are encoded by Slc23a1 and Slc23a2 genes, respectively.
  • Understanding the roles of SVCT isoforms in ascorbate homeostasis is critical.

Purpose of the Study:

  • To investigate the roles of SVCT1 and SVCT2 in regulating ascorbate concentrations in adult mice.
  • To determine the tissue-specific expression patterns of SVCT1 and SVCT2.
  • To examine the influence of gender on ascorbate levels and transport.

Main Methods:

  • Utilized Slc23a2+/- and Slc23a2+/+ mice on an ascorbate-free diet.
  • Collected 24-h urine samples for analysis of ascorbate excretion and creatinine ratio.

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  • Employed RT-PCR to assess SVCT1 and SVCT2 mRNA expression in various tissues.
  • Measured ascorbate concentrations in plasma and specific tissues.

    • Main Results:

    • SVCT1 predominantly expressed in liver and kidney; SVCT2 in brain, skeletal muscle, and spleen.
    • Slc23a2+/- mice showed reduced SVCT2 but normal SVCT1 mRNA levels.
    • Ascorbate concentrations were lower in Slc23a2+/- mice in SVCT2-predominant tissues.
    • Females exhibited lower ascorbate excretion and higher plasma/SVCT1-tissue ascorbate concentrations compared to males.
    • SVCT2 influenced gender-specific spleen ascorbate levels.

    Conclusions:

    • SVCT2 is a key determinant of ascorbate accumulation in tissues lacking SVCT1.
    • SVCT isoforms appear to function independently.
    • Lower ascorbate excretion in females may contribute to higher vitamin C levels in plasma and SVCT1-expressing tissues.