Iron absorption from NaFeEDTA is downregulated in iron-loaded rats

NaFeEDTA is a promising fortificant for use in plant foods, because it is less susceptible to iron absorption inhibitors and has fewer undesirable impacts on sensory quality than ferrous sulfate. However, the hypothesis that iron absorption from NaFeEDTA is effectively downregulated in iron-overload conditions has not been thoroughly tested. Therefore, the objective of this study was to compare downregulation of iron absorption from ferrous sulfate and NaFeEDTA in intact iron-loaded rats. Male Sprague-Dawley rats were fed diets containing either ferrous sulfate (35 mg Fe per 1 kg diet) or elemental iron (30,000 mg Fe per 1 kg diet) for 29 d to achieve basal or iron-loaded status. While body weights and hemoglobin concentrations were the same for basal and iron-loaded rats, nonheme-iron concentrations in liver, spleen, and kidney were all significantly higher in iron-loaded rats, indicating elevated iron status. Percentage of iron absorption from (59)Fe-labeled ferrous sulfate and NaFeEDTA, determined from whole-body retention of (59)Fe activity, was 64.7 and 49.4% in basal rats but decreased to 12.8 and 10.2% in iron-loaded rats, respectively. The reductions in percentage of iron absorption from both iron sources in rats as a result of iron loading were comparable (about -80% for both iron sources). Our results suggest that iron absorption from NaFeEDTA and ferrous sulfate is downregulated to a similar extent in iron-loaded rats. Hence, NaFeEDTA is no more likely than ferrous sulfate to exacerbate iron overload in subjects with adequate body iron stores.