Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nutritional regulation of mRNA processing.

Lisa M Salati1, Wioletta Szeszel-Fedorowicz, Huimin Tao

  • 1Department of Biochemistry and Molecular Pharmacology, School of Medicine, West Virginia University, Morgantown, WV, USA. Lsalati@hsc.wvu.edu

The Journal of Nutrition
|August 31, 2004
PubMed
Summary

Dietary nutrients regulate cell energy metabolism by controlling enzyme synthesis. Glucose-6-phosphate dehydrogenase (G6PD) gene expression is uniquely controlled post-transcriptionally, specifically via mRNA splicing, offering new insights into nutrient-mediated gene regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spinal phosphaturic mesenchymal tumor: a case report of thoracic vertebral involvement with preoperative embolization and literature review.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Ondansetron exhibits potent antiviral activity against enterovirus 71 infection in vitro and in vivo.

Virus research·2026
Same author

Integrating serological markers of Helicobacter pylori and pepsinogen to improve detection of gastric precancerous conditions.

BMC gastroenterology·2026
Same author

Engineered biomimetic exosomes for small activating RNA delivery: Low-density lipoprotein receptor-related protein-1 targeted blood-brain barrier penetration and dual therapy in intracerebral Hemorrhage.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

p75 neurotrophin receptor exacerbates neuronal apoptosis after intracerebral hemorrhage by promoting OTUB1 ubiquitination and degradation.

Brain research bulletin·2026
Same author

Research on the Microstructure and Performance Regulation of SLM 304 Steel Under Intermittent Deformation.

Materials (Basel, Switzerland)·2026

Area of Science:

  • Cellular metabolism
  • Molecular biology
  • Nutritional biochemistry

Background:

  • Cellular adaptation to dietary energy sources (carbohydrates vs. triacylglycerols) depends on regulating lipogenic enzyme activity.
  • Enzyme activity is primarily modulated by changes in protein synthesis rates, influenced by dietary nutrients via hormonal or direct signaling pathways.
  • Most lipogenic genes are transcriptionally regulated, but Glucose-6-phosphate dehydrogenase (G6PD) exhibits unique post-transcriptional control.

Purpose of the Study:

  • To elucidate the post-transcriptional regulatory mechanisms governing Glucose-6-phosphate dehydrogenase (G6PD) synthesis in response to dietary cues.
  • To investigate how dietary carbohydrates and polyunsaturated fats differentially affect G6PD gene expression at the post-transcriptional level.

Main Methods:

Related Experiment Videos

  • Review of evidence on the nutritional regulation of G6PD synthesis.
  • Analysis of post-transcriptional control mechanisms, focusing on mRNA splicing efficiency.
  • Identification of cis-acting sequences involved in nutrient-mediated regulation of G6PD gene expression.

Main Results:

  • Nutritional regulation of G6PD synthesis occurs exclusively at the post-transcriptional level.
  • Dietary carbohydrates enhance G6PD activity, while polyunsaturated fats inhibit it.
  • Regulation involves altered splicing efficiency of the nascent G6PD transcript, influenced by a cis-acting sequence in exon 12, particularly for polyunsaturated fat inhibition.

Conclusions:

  • G6PD represents a unique model for studying nutrient-regulated gene expression at the post-transcriptional level.
  • Dietary fat and carbohydrate intake significantly impact G6PD mRNA processing and synthesis.
  • Understanding these mechanisms provides novel insights into the breadth of gene regulation by nutrients.