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Updated: Aug 22, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Quantifying anti-HBV effect of targeted ribonuclease by real-time fluorescent PCR
Jun Liu1, Ying-Hui Li, Jin Ding
1Department of Etiology, Fourth Military Medical University, Xi'an 710033, Shaanxi Province, China. etiology@fmmu.edu.cn
Aim:
To quantify the inhibition of HBV replication by targeted ribonuclease by using real-time fluorescent PCR.
Methods:
Targeted ribonuclease was introduced into 2.2.15 cells by liposome-mediated transfection or HIV-TAT mediated protein transduction. Forty-eight hours after the transfection and 24 h after the transduction, supernatants of 2.2.15 cells were collected and HBV DNA in the supernatants was quantified by real-time fluorescent PCR with a commercial kit.
Results:
HBV DNA concentrations in the supernatants of 2.2.15 cells transfected or transducted with targeted ribonuclease were 4.9+/-2.4 x 10(8) copies/L and 8.3+/-4.0 x 10(8) copies/L, respectively. Compared with controls, transfection or transduction of targeted ribonuclease reduced HBV DNA concentration in the supernatants of 2.2.15 cells by 90.4% and 90.1%, respectively (P<0.05).
Conclusion:
Targeted ribonuclease can inhibit HBV replication in 2.2.15 cells.
