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Related Experiment Videos

Does oligodendrocyte survival depend on axons?

B A Barres1, M D Jacobson, R Schmid

  • 1Medical Research Council Developmental Neurobiology Programme, Department of Biology, Medawar Building, University College, London WC1E 6BT, UK.

Current Biology : CB
|August 1, 1993
PubMed
Summary
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Oligodendrocyte survival in developing nerves depends on axons. Axon degeneration causes oligodendrocyte death, which can be prevented by survival factors like CNTF and IGF-1.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Oligodendrocytes and their precursors require cell-derived signals for survival in culture.
  • Approximately 50% of developing oligodendrocytes in the rat optic nerve undergo cell death, suggesting competition for limited survival factors.
  • Axons may regulate oligodendrocyte survival factors, matching oligodendrocyte numbers to myelination needs.

Purpose of the Study:

  • To test the hypothesis that developing oligodendrocyte survival is dependent on axons.
  • To investigate the role of axons in regulating oligodendrocyte numbers during development.

Main Methods:

  • Examining oligodendrocyte death in transected rat optic nerves with degenerating axons.
  • Assessing the effects of exogenous ciliary neurotrophic factor (CNTF) and insulin-like growth factor I (IGF-1) on oligodendrocyte survival.

Related Experiment Videos

  • Investigating the survival of purified oligodendrocytes in the presence of purified neurons in vitro.

    • Main Results:

    • Oligodendrocyte death was observed specifically in transected nerves where axons degenerated.
    • Exogenous administration of CNTF or IGF-1 prevented oligodendrocyte cell death in transected nerves.
    • Purified neurons were found to promote the survival of purified oligodendrocytes in vitro.

    Conclusions:

    • Oligodendrocyte survival is strongly dependent on the presence of axons.
    • These findings support the hypothesis that competition for axon-derived survival signals regulates oligodendrocyte numbers for myelination.
    • The specific molecular identities of these axon-dependent survival signals require further investigation.