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Updated: Aug 22, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
Published on: December 10, 2016
The cme gene of Clostridium difficile confers multidrug resistance in Enterococcus faecalis
Sacha Lebel1, Sylvie Bouttier, Thierry Lambert
1Faculté de Pharmacie, Département de Microbiologie, Unité Ecosystème Microbien Digestif et Santé, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France. sacha.lebel@cep.u-psud.fr
Abstract:
Antibiotic resistance in C. difficile by efflux has been previously suggested. The genome of C. difficile 630 was screened for sequences encoding putative proteins homologous to NorA from Staphylococcus aureus. Four ORFs homologous to efflux genes were cloned into the pAT79 shuttle vector under the control of transcription and translation signals of Gram-positive bacteria and expressed in Enterococcus faecalis JH2-2 and S. aureus RN4220. One of these sequences, designated cme conferred resistance to ethidium bromide, safranin O, and erythromycin in E. faecalis. The three other ORFs did not confer detectable resistance in both bacteria.
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