Related Experiment Video
Updated: Aug 22, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Diversity of 16S rDNA sequences of Rhizobium spp. implications for species determinations
John M Young1, Duck-Chul Park, Bevan S Weir
1Landcare Research, Private Bag 92170, Auckland, New Zealand. youngj@LandcareResearch.co.nz
Abstract:
Comparative analysis of 70 16S rDNA sequences representing 20 Rhizobium species (including pathogenic Agrobacterium spp.) was conducted using Maximum Likelihood to establish relationships of species using multiple sequences. There is no significant internal division of the Rhizobium clade to suggest that it represents more than one genus. Plant pathogenic (Agrobacterium) species are distributed within the genus. The analysis supported the synonymy of some species (Rhizobium gallicum and Rhizobium mongolense) and the need for comparative investigations of the tumorigenic and nodulating properties of Rhizobium tropici and Rhizobium rhizogenes. Misidentification of some sequences may conceal one or more putative novel species. Some sequences appear to be misidentified because of faulty sequencing or incomplete or inadequate analysis.
Related Concept Videos
Modern Molecular Taxonomy
Diversity of Protists III
Applications of Molecular Taxonomy
Phylogenetic Species Concept in Microbiology
Evolutionary Relationships through Genome Comparisons
Methods of Classification and Identification

