Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of KIT (CD117) expression in gallbladder carcinomas by tissue microarray.

C Langner1, M Lemmerer, P Kornprat

  • 1Institute of Pathology, Medical University of Graz, Auenbruggerplatz 25, A-8036 Graz, Austria. cord.langner@meduni-graz.at

European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology
|September 1, 2004
PubMed
Summary

KIT (CD117) is rarely found in gallbladder carcinomas. This study found KIT immunoreactivity in only three tumors, suggesting it is not a viable target for targeted therapies like imatinib mesylate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Value of sarcopenia in the resection of colorectal liver metastases-a systematic review and meta-analysis.

Frontiers in oncology·2023
Same author

Identification and validation of fusidic acid and flufenamic acid as inhibitors of SARS-CoV-2 replication using DrugSolver CavitomiX.

Scientific reports·2023
Same author

Use of LigaSure vessel sealing system versus conventional axillary dissection in breast cancer patients: a retrospective comparative study.

BMC surgery·2022
Same author

Metachronous hepatic resection for liver only pancreatic metastases.

Surgical oncology·2020
Same author

Prophylactic salpingectomy for prevention of ovarian cancer at the time of elective laparoscopic cholecystectomy.

The British journal of surgery·2020
Same author

Non-obstetric surgery during pregnancy - an eleven-year retrospective analysis.

BMC pregnancy and childbirth·2019

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • KIT (CD117) is a receptor tyrosine kinase implicated in cell growth and survival.
  • KIT's presence can indicate a potential target for cancer therapies.
  • Gallbladder carcinomas are a group of aggressive cancers with limited treatment options.

Purpose of the Study:

  • To investigate the expression of KIT in primary and metastatic gallbladder carcinomas.
  • To determine if KIT is a potential therapeutic target in gallbladder cancer.

Main Methods:

  • Tissue microarray technique was used to analyze 57 primary gallbladder carcinomas and 18 metastases.
  • Immunohistochemistry staining was performed using two distinct KIT antibodies (polyclonal and monoclonal).

Related Experiment Videos

Main Results:

  • KIT expression was infrequent, observed in only 3 out of 57 primary gallbladder carcinomas.
  • One tumor showed reactivity with a polyclonal antibody (well-differentiated papillary adenocarcinoma).
  • Two tumors showed weak, focal reactivity with a monoclonal antibody (poorly differentiated tubular adenocarcinoma).

Conclusions:

  • KIT immunoreactivity is uncommon in gallbladder carcinoma.
  • Routine immunohistochemical screening for KIT is not cost-effective.
  • The low prevalence of KIT does not support its use as a therapeutic target or for imatinib mesylate trials in advanced gallbladder cancer.