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Published on: September 23, 2015
Distribution and MK-801-induced expression of serine racemase mRNA in rat brain by real-time quantitative PCR
Masanobu Yoshikawa1, Tomomi Kobayashi, Tetsuo Oka
1Department of Pharmacology, School of Medicine, Tokai University, Isehara, Kanagawa 259-1143, Japan.
Abstract:
We have used real-time quantitative PCR methods to evaluate the effect of the systemic administration of (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine (MK-801; 0.4 mg/kg) on the gene expression of serine racemase in several brain areas of the rat. The levels of serine racemase mRNA in all the brain areas transiently increased after the administration. The present findings suggest that there is a link between the expression of serine racemase mRNA and the activity of the NMDA receptors.
