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Related Experiment Videos

What turns CREB on?

Mona Johannessen1, Marit Pedersen Delghandi, Ugo Moens

  • 1Department of Biochemistry, Institute of Medical Biology, University of Tromsø, N-9037, Norway.

Cellular Signalling
|September 1, 2004
PubMed
Summary
This summary is machine-generated.

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The cAMP response element-binding protein (CREB) has distinct domains for basal and signal-induced transcription. While Ser-133 phosphorylation is key, other mechanisms also regulate CREB activity, explaining varied responses.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Signal Transduction

Background:

  • The cAMP response element-binding protein (CREB) is a transcription factor crucial for regulating gene expression.
  • CREB's transactivation domain comprises two key regions: the glutamine-rich Q2 domain and the kinase-inducible domain.
  • The Q2 domain facilitates basal transcription by interacting with TAFII130/135 and recruiting RNA polymerase II.

Purpose of the Study:

  • To review stimuli that induce CREB phosphorylation at Ser-133.
  • To explore both Ser-133-dependent and -independent mechanisms influencing CREB-mediated transcription.
  • To discuss models explaining the dissociation between CREB Ser-133 phosphorylation and transcriptional activation.

Main Methods:

  • Literature review of studies on CREB phosphorylation and transcriptional regulation.

Related Experiment Videos

  • Analysis of diverse stimuli impacting CREB signaling pathways.
  • Comparison of models for CREB activation and coactivator recruitment (e.g., CBP, p300).

    • Main Results:

    • Signal-induced phosphorylation at Ser-133 is generally considered essential for robust CREB activation via coactivator recruitment.
    • However, CREB activity can be modulated by mechanisms independent of Ser-133 phosphorylation.
    • Not all stimuli that trigger Ser-133 phosphorylation lead to increased CREB-dependent transcription, indicating complex regulatory layers.

    Conclusions:

    • CREB-mediated transcription involves intricate regulation beyond simple Ser-133 phosphorylation.
    • Understanding both dependent and independent pathways is crucial for deciphering CREB's diverse roles in cellular responses.
    • Further research is needed to fully elucidate the discrepancies observed between CREB phosphorylation status and transcriptional output.