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A suppressive lymphokine derived from Ts clone 13G2 is IL-10
T Hisatsune1, Y Minai, K Nishisima
1Department of Agricultural Chemistry, University of Tokyo, Japan.
Lymphokine and Cytokine Research
|April 1, 1992
Summary
CD8+ suppressor T cells produce a factor that inhibits helper T-cell proliferation. This T-cell growth inhibitory factor (TGIF) was identified as Interleukin-10 (IL-10), highlighting IL-10's role in immune suppression.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD8+ suppressor T cells (Ts) play a crucial role in regulating immune responses.
- These cells can produce soluble factors that modulate the activity of other immune cells.
Purpose of the Study:
- To characterize a suppressive lymphokine produced by a CD8+ Ts clone (13G2).
- To identify the molecular nature of this T-cell growth inhibitory factor (TGIF).
Main Methods:
- Sequential chromatography was used to purify TGIF from cell culture supernatants.
- Neutralization assays with anti-IL-10 monoclonal antibody were performed.
- Immunoblotting and PCR were employed to detect IL-10 and its mRNA.
Main Results:
- TGIF was purified and demonstrated potent suppression of type 1 helper T-cell (Th1) proliferation.
- The suppressive activity of TGIF was neutralized by anti-IL-10 antibody and replaced by IL-10.
- IL-10 protein and mRNA were detected in the purified TGIF and the producing cells, respectively.
Conclusions:
- The T-cell growth inhibitory factor (TGIF) produced by CD8+ T-cell clone 13G2 is identified as Interleukin-10 (IL-10).
- IL-10 plays a significant role in the immune suppressive functions of Ts cells.