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Related Experiment Videos

Unexpectedly complex editing patterns at dinucleotide insertion sites in Physarum mitochondria.

Elaine M Byrne1, Jonatha M Gott

  • 1Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106, USA.

Molecular and Cellular Biology
|September 2, 2004
PubMed
Summary

RNA editing in Physarum polycephalum involves inserting non-encoded nucleotides. This study shows both single and double nucleotide insertions occur during transcription, suggesting a unified editing process.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Mitochondrial RNAs in Physarum polycephalum undergo extensive RNA editing.
  • Editing primarily involves insertion of non-encoded nucleotides, with some substitutional editing (C to U).
  • Previous work indicated single nucleotide insertions are cotranscriptional.

Purpose of the Study:

  • To investigate the timing of both single and dinucleotide insertions into mitochondrial RNAs.
  • To determine if dinucleotide insertions occur during or after transcription.
  • To analyze the patterns of nucleotide addition in nascent RNAs.

Main Methods:

  • In vitro and in vivo synthesis of nascent RNAs.
  • Analysis of cloned complementary DNAs (cDNAs) derived from edited RNAs.

Related Experiment Videos

  • Examination of nucleotide insertion patterns and partial editing events.

    • Main Results:

    • The distribution of inserted nucleotides supports cotranscriptional addition for all insertion sites, including dinucleotides.
    • Patterns of partial editing and misediting suggest distinct factors may be involved in specific dinucleotide insertions.
    • Evidence suggests nucleotides might be added and subsequently modified post-transcriptionally.

    Conclusions:

    • RNA editing by nucleotide insertion in Physarum polycephalum mitochondria is largely a cotranscriptional process.
    • Specific dinucleotide insertion sites may require unique editing factors.
    • Post-transcriptional modification of inserted nucleotides is a possibility.