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Related Experiment Videos

The knockout mouse project.

Christopher P Austin1, James F Battey, Allan Bradley

  • 1National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA. austinc@mail.nih.gov

Nature Genetics
|September 2, 2004
PubMed
Summary

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This summary is machine-generated.

Creating a comprehensive, publicly available collection of mouse knockouts is crucial for advancing biomedical research. This high-throughput effort will standardize gene function studies and accelerate biological discovery.

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Biomedical Research

Background:

  • Mouse knockout technology is essential for understanding gene function in vivo.
  • Currently, only about 10% of mouse genes have published knockouts.
  • Existing knockouts often lack standardization in creation and phenotyping, limiting their utility and availability.

Purpose of the Study:

  • To advocate for a global, high-throughput initiative to generate knockouts for all mouse genes.
  • To emphasize the need for standardized phenotyping and public accessibility of these genetic resources.
  • To accelerate biomedical discovery through comprehensive and accessible mouse knockout models.

Main Methods:

  • Leveraging new technologies and production efficiencies for high-throughput knockout generation.

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  • Implementing standardized phenotyping protocols across all generated knockouts.
  • Establishing a public domain repository for all mouse knockout resources.

    • Main Results:

    • A significant gap exists in the availability of characterized mouse knockouts for most genes.
    • Current resources are often not standardized or publicly accessible, hindering research.
    • A coordinated international effort is proposed to overcome these limitations.

    Conclusions:

    • A genome-wide collection of standardized, publicly available mouse knockouts is needed.
    • This resource will significantly enable and accelerate biomedical discovery.
    • Harnessing new technologies is key to achieving this goal efficiently.