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Updated: Aug 14, 2026
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
[Development of recombinant human-derived IgG antibody against HBsAg]
Yu Guo1, Mi-fang Liang, Sheng-li Bi
1Laboratory of Hepatitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Background:
To investigate preparation of human-derived anti-HBs IgG antibody.
Methods:
mRNA was extracted from lymphocytes of a volunteer with high level antibody to HbsAg. Fab phage display library was constructed. Purified HBsAg was used to screen the Fab. Eukaryotic expression vector was constructed and transfected into sf9 cells, then the supernatant was purified. The expression of anti-HBs human IgG was tested with blocking ELISA and immunofluorescent test.
Results:
A whole human anti-HBs IgG molecule was obtained, the antibody reacts with HBsAg "a" determinant.
Conclusion:
It can be concluded preliminarily that this antibody has neutralizing characteristic against HbsAg.
