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[Nucleotides sequence analysis of EV71 Chinese strain SHZH98 3C segment]
Ya-qing He1, Fan Yang, Liang-cheng Li
1Department of Microbiology, Shenzhen Centen Center for Disease Control and Prevention, Shenzhen 518020, China.
Summary
The 3C gene of Enterovirus 71 (EV71) Chinese strain SHZH98 shows greater genetic similarity to European and American strains than to Taiwan strains. This suggests a distinct evolutionary path for EV71 non-structural proteins in China.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Context:
- Enterovirus 71 (EV71) is a significant human pathogen.
- Understanding EV71 genetic diversity is crucial for public health.
- The 3C gene plays a vital role in EV71 replication and evolution.
Purpose:
- To analyze the genetic evolution of the EV71 Chinese strain SHZH98.
- To use the 3C gene as a molecular marker for evolutionary studies.
- To compare the genetic characteristics of the Chinese EV71 strain with other global strains.
Summary:
- The 3C gene cDNA of EV71 Chinese strain SHZH98 was amplified and sequenced.
- Nucleotide sequence analysis revealed higher homology of the SHZH98 strain to European/American strains (e.g., MS, BrCr) than to Taiwan strains (e.g., POLY, NCKU).
- The deduced amino acid sequences also showed a similar pattern, with higher homology to MS (93.45%) and BrCr (89.1%) compared to Taiwan strains.
Impact:
- Provides insights into the evolutionary dynamics of EV71 in China.
- Suggests that EV71 non-structural proteins in Chinese strains may have evolved differently from those in Taiwan strains.
- Contributes to the understanding of EV71 genetic diversity and potential origins.