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Updated: Aug 22, 2026
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
[Preliminary studies on pathogenic factors of human cytomegalovirus infection]
Ai-bin Li1, Qian Liu, Liang-bin Xia
1The Ren Min Hospital of Wu Han University, Wuhan 430060, China.
Objective:
To investigate the pathogenic factors of human cytomegalovirus (HCMV) infections.
Methods:
Totally 36 serum samples were obtained from early pregnant woman and examined with ELISA for anti-HCMV antibody IgG and IgM. After artificial abortion,chorionic villus and decidua were also examined with polymerase chain reaction (PCR) for HCMV-DNA. When the results of PCR were positive, pathological changes of these chorionic villus and decidua were analyzed.
Results:
The results showed that only 10 samples were PCR positive while IgG and/or IgM antibody to HCMV was positive. After infection with HCMV, different changes occurred in chorionic villus and decidual trophoblastic cells placental villus were hyperplasic and decidua cells degenerated and necrotized followed by lymphocytes infiltration.
Conclusion:
These pathological changes may be one of pathogenic factors of HCMV.
