Study on noncovalent complexes of cytotoxic protoberberine alkaloids with double-stranded DNA by using electrospray
Wen-Hua Chen1, Chi-Leung Chan, Zongwei Cai
1School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Abstract:
The noncovalent complexes of four cytotoxic protoberberine alkaloids that is, berberine, palmatine, jatrorrhizine, and coptisine with double-stranded oligodeoxynucleotides d(AAGAATTCTT)(2) were investigated by electrospray ionization mass spectrometry. These four active components from Chinese herbal medicines showed both 1:1 and 1:2 binding stoichiometries, independent on the alkaloid-to-DNA ratios. Binding affinities in the order of palmatine> or =jatrorrhizine>coptisine>berberine with d(AAGAATTCTT)(2) were obtained. Additionally, the preliminary results indicated that berberine had some sequence selectivities.
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