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Related Experiment Videos

Inducible carboxypeptidase activity in vancomycin-resistant enterococci.

L Gutmann1, D Billot-Klein, S al-Obeid

  • 1Laboratoire de Microbiologie Médicale, Université Paris VI, France.

Antimicrobial Agents and Chemotherapy
|January 1, 1992
PubMed
Summary

Vancomycin coinduces DD-carboxypeptidase activity and resistance in enterococci. This enzyme

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Enterococci exhibit resistance to glycopeptide antibiotics like vancomycin.
  • The mechanisms underlying vancomycin resistance in enterococci are not fully elucidated.
  • DD-carboxypeptidase activity has been implicated in bacterial cell wall metabolism.

Purpose of the Study:

  • To investigate the role of DD-carboxypeptidase activity in vancomycin resistance in enterococci.
  • To determine if vancomycin can induce DD-carboxypeptidase activity.
  • To characterize the enzymatic properties of the induced DD-carboxypeptidase.

Main Methods:

  • Testing eight strains of enterococci for vancomycin-induced resistance and DD-carboxypeptidase activity.
  • Analyzing a constitutively resistant mutant (MT10) of Enterococcus faecium (D366).

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  • In vitro enzymatic assays using various substrates and inhibitors.

    • Main Results:

    • Vancomycin coinduced DD-carboxypeptidase activity and resistance in susceptible enterococcal strains.
    • A resistant mutant (MT10) exhibited spontaneous carboxypeptidase activity.
    • The enzyme utilized pentapeptide, UDP-MurNac-pentapeptide, and D-alanyl-D-alanine as substrates and was penicillin-insensitive.
    • A correlation was observed between vancomycin resistance levels and carboxypeptidase activity.

    Conclusions:

    • DD-carboxypeptidase is a key component of the vancomycin resistance mechanism in enterococci.
    • The enzyme's activity is linked to the modification of peptidoglycan precursors.
    • Understanding this mechanism could inform strategies to combat antibiotic resistance.