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Related Experiment Videos

Spreading depression activates unfolded protein response.

Edda Schneeloch1, Simone Wenkel, Günter Mies

  • 1Laboratory of Molecular Neurobiology, Max-Planck-Institute for Neurological Research, Cologne, Gleuelerstr. 50, 50931 Koeln, Germany.

Neuroscience Letters
|September 3, 2004
PubMed
Summary

Preconditioning using repetitive spreading depression activates the unfolded protein response (UPR). This UPR activation may protect cells by preserving endoplasmic reticulum (ER) function during stress.

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Area of Science:

  • Neuroscience
  • Cellular Biology
  • Stress Response Mechanisms

Background:

  • Preconditioning involves non-lethal stress activating protective responses against lethal stress.
  • Methods like ischemia or spreading depression can induce preconditioning.
  • The unfolded protein response (UPR) is activated by endoplasmic reticulum (ER) dysfunction.

Purpose of the Study:

  • To investigate the effect of repetitive spreading depression on the UPR.
  • To determine if spreading depression activates UPR signaling pathways.

Main Methods:

  • Induction of repetitive spreading depression for 1 hour.
  • Analysis of x-box binding protein 1 (xbp1) mRNA processing.
  • Measurement of glucose-regulated protein 78 (grp78) mRNA levels.

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Main Results:

  • Repetitive spreading depression induced processing of xbp1 mRNA, indicating UPR activation.
  • GRP78 mRNA levels increased, suggesting activation of a protein synthesis-dependent pathway.
  • These findings demonstrate that spreading depression activates the UPR.

Conclusions:

  • Preconditioning with spreading depression activates the UPR.
  • Activated UPR may maintain ER function under pathological conditions.
  • This study highlights a potential protective mechanism against ER dysfunction.