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Updated: Aug 22, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Dioleoyl phosphatidylethanolamine and PEG-lipid conjugates modify DNA delivery mediated by 1,4-dihydropyridine
Zanna Hyvönen1, Seppo Rönkkö, Marjo-Riitta Toppinen
1Department of Pharmaceutics, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. zanna.hyvonen@uku.fi
Abstract:
Complexes of amphiphilic cationic 1,4-dihydropyridines with DNA (lipoplexes) can be used for nonviral gene delivery. In order to achieve serum-resistant transfection system, DOPE and PEG-lipid conjugates were used to modify 1,4-dihydropyridine amphiphile DHP-12 complexes with DNA. The ability to bind DNA was examined by ethidium bromide displacement assay. Cellular uptake, transfection efficacy and intracellular trafficking of the lipoplexes were assessed using FACS, betagalactosidase gene transfection and confocal laser microscopy, respectively. Cytotoxicity was determined by MTT assay. DHP-12 lipoplexes that included DOPE showed enhanced cell uptake and transfection efficacy both in the absence and presence of serum. PEG-lipid conjugates, in contrast, impaired transfection. In conclusion, combination of DHP-12 with DOPE appears to be a promising transfection system.
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