Related Experiment Video

Updated: Aug 18, 2026

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
07:39

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

[Observation of T cells and T-cell subsets in mycosis fungoides]

X Zeng1

  • 1Institute of Dermatology, Nanjing.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
|February 1, 1992
PubMed

Skin lesions from 14 patients with mycosis fungoides (MF) were studied immunohistochemically with monoclonal antibodies that identify pan T (Leu4), T-helper/inducer (TH), T-suppressor/cytotoxic (Ts) (Leu3a, Leu2a) and pan B (Leu12) cells. Lymphoid cell reactivity was diverse among these cases. Among 12 cases of MF, TH cells were predominant over Ts cells, and one case featured nonreactive Ts cells in the dermis. In one case of MF, Ts cells were predominant. One case of MF showed strong reactivity for both T cell subset markers. The intraepidermal lymphoid cellularity was Leu4-reactive in 14 cases of MF. Among these 14 cases, the intraepidermal cellularity was Leu3a-reactive in 11 and Leu2a-reactive in 4. The use of such studies of T cell subsets may demonstrate a correlation of MF with cytomorphology, clinical stage, and disease prognosis.

More Related Videos

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling09:08

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells09:04

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Published on: March 7, 2025

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...