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Related Experiment Videos

Functional innervation in tissue engineered models for in vitro study and testing purposes.

Erik J Suuronen1, Christopher R McLaughlin, Peter K Stys

  • 1University of Ottawa Eye Institute, Ottawa Health Research Institute--Vision Centre, and Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, K1H 8L6, Canada.

Toxicological Sciences : an Official Journal of the Society of Toxicology
|September 3, 2004
PubMed
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Innervation significantly improves engineered human cornea tissue function and protects the epithelium. Nerves in engineered tissues can also indicate the severity of chemical damage.

Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Neuroscience

Background:

  • Tissue engineering aims to create functional tissue substitutes.
  • Simulated tissues offer advanced models for toxicology and nerve studies.
  • Innervation's role in engineered tissue function is not fully understood.

Purpose of the Study:

  • To investigate the importance of innervation in engineered human cornea models.
  • To assess how nerves influence epithelial growth, mucin layer formation, and protection from chemical insult.
  • To explore the use of nerve responses as indicators of tissue damage.

Main Methods:

  • Development of a tissue-engineered human cornea model with innervation.
  • Utilizing 2-photon microscopy to measure intracellular sodium changes in nerves.

Related Experiment Videos

  • Assessing epithelial cell death and mucin layer formation post-chemical insult.

    • Main Results:

    • Innervation enhanced epithelial growth and mucin layer formation in the engineered cornea.
    • Nerve presence protected the epithelium from chemical-induced cell death.
    • Differential nerve responses to chemical stimuli were observed via sodium changes.
    • 2-photon microscopy visualized sensory axon fibers within the 3D tissue.

    Conclusions:

    • Innervation plays a crucial role in the protective qualities and function of engineered tissues.
    • Engineered nerves can serve as sensitive indicators of chemical insult severity.
    • These findings are vital for developing optimized tissue-engineered models for in vitro research and testing.