Insulin rapidly stimulates binding of phosphofructokinase and aldolase to muscle cytoskeleton

1. We report here on a novel action of insulin which shows that the hormone stimulates binding of phosphofructokinase (PFK) and aldolase to muscle cytoskeleton. 2. This effect was demonstrated both in vivo, by injection of insulin, in the tibialis anterior and gastrocnemius muscles, as well as in vitro, in the isolated rat diaphragm muscle incubated with insulin. 3. Insulin exerted this effect at physiologic range of concentrations and very rapidly (about 50% stimulation of binding occurred within 1 min). 4. The possible physiological significance of this rapid action of insulin, is to provide local ATP, generated by the accelerated cytoskeletal glycolysis, for other rapidly insulin-stimulated membrane-cytoskeleton processes.