Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simultaneous topography and recognition imaging using force microscopy.

Cordula M Stroh1, Andreas Ebner, Manfred Geretschläger

  • 1Institute for Biophysics, Atomic Physics and Surface Science, Johannes Kepler University of Linz, A-4040 Linz, Austria.

Biophysical Journal
|September 4, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization of Culture Conditions for Pyrroloquinoline Quinone-Overproducing Mutant <i>Hyphomicrobium denitrificans</i> and Its Skin Bioactive Properties.

Journal of microbiology and biotechnology·2026
Same author

Single molecule force spectroscopy for evaluating inhibitors of SARS-CoV-2 variants of concern.

European biophysics journal : EBJ·2026
Same author

Comparative Analysis of Two Measurement Modalities for Ex Vivo Analysis of Corneal Stiffness in Porcine Corneas.

Bioengineering (Basel, Switzerland)·2025
Same author

Anti-CD38 VLRB-Fc antibodies from transgenic tobacco exhibit potent anti-myeloma activity in vitro and in vivo.

International journal of biological macromolecules·2025
Same author

Nanomechanical binding mechanism of ligands drives agonistic activity.

Nature communications·2025
Same author

Revealing the location and dynamics of a concealed binding site in the dopamine transporter.

Nature communications·2025

This study introduces a new method combining dynamic force microscopy and single molecule recognition force spectroscopy for high-accuracy imaging. It simultaneously maps surface topography and specific binding sites with nanometer precision.

Area of Science:

  • Biophysics
  • Nanotechnology
  • Surface Science

Background:

  • Accurate simultaneous imaging of surface topography and molecular binding is crucial for understanding biological and material interfaces.
  • Existing methods often face limitations in spatial resolution or the ability to distinguish between topographic and recognition signals.

Purpose of the Study:

  • To develop and demonstrate a novel method for simultaneous recording of topography and specific binding sites with nanometer positional accuracy.
  • To overcome the challenge of separating topographic and recognition signals in high-resolution imaging.

Main Methods:

  • Combining dynamic force microscopy (DFM) with single molecule recognition force spectroscopy.
  • Utilizing magnetically oscillated AFM tips functionalized with antibodies.

Related Experiment Videos

  • Employing a specially designed electronic circuit to differentiate cantilever oscillation maxima and minima.

    • Main Results:

    • Simultaneous acquisition of topography and recognition images with nanometer positional accuracy (Δz ≈ 5 nm).
    • Effective separation of topographic and recognition signals in both space and time (≈ 0.1 ms).
    • Demonstrated the method using lysozyme adsorbed to mica.

    Conclusions:

    • The developed method enables precise localization of specific binding sites alongside surface topography mapping.
    • This technique offers a significant advancement for high-resolution surface analysis and molecular interaction studies.
    • The approach provides a robust platform for investigating complex surface phenomena at the nanoscale.