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Related Experiment Videos

Phosphoproteomic analysis of the developing mouse brain.

Bryan A Ballif1, Judit Villén, Sean A Beausoleil

  • 1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Molecular & Cellular Proteomics : MCP
|September 4, 2004
PubMed
Summary
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Researchers profiled protein phosphorylation sites in the developing mouse brain using mass spectrometry. This study identified over 500 phosphorylation sites, advancing our understanding of brain development regulation.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Mammalian brain development relies on precise, regulated stimuli.
  • Signal transduction often involves reversible protein phosphorylation.
  • Neuronal signaling can generate complex phosphoprotein networks.

Purpose of the Study:

  • To develop a method for global phosphoprotein profiling in developing brain tissue.
  • To perform the first proteomic-scale phosphorylation site analysis on primary animal tissue.

Main Methods:

  • Utilized a mass spectrometry-based approach.
  • Applied the methodology to analyze the developing mouse brain proteome.

Main Results:

  • Identified over 500 protein phosphorylation sites.

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  • Enabled proteomic-scale analysis of phosphorylation in primary tissue.

    • Conclusions:

    • The developed mass spectrometry method is effective for large-scale phosphoproteomic analysis.
    • This work provides a foundational dataset for understanding regulatory mechanisms in brain development.