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A percutaneous device to study glucose kinetics in subcutaneous tissue fluid
M Gerritsen1, J A Lutterman, J A Jansen
1Department of Biomaterials, University of Nijmegen, 6500 HB Nijmegen, The Netherlands.
Journal of Materials Science. Materials in Medicine
|September 7, 2004
Summary
This study shows that percutaneous devices can measure subcutaneous glucose levels, but porous membranes hinder accuracy. Uncovered devices accurately reflect blood glucose changes, making them suitable for glucose sensor research.
Area of Science:
- Biomedical Engineering
- Endocrinology
- Metabolic Research
Background:
- Accurate monitoring of subcutaneous glucose is crucial for diabetes management.
- Percutaneous devices offer a potential method for in vivo glucose measurement.
- Understanding the influence of implant materials on glucose kinetics is essential.
Purpose of the Study:
- To investigate subcutaneous glucose kinetics using a percutaneous device (PD) in rabbits.
- To evaluate the impact of different membrane coverings on glucose measurement accuracy.
- To assess the suitability of PDs for modeling implantable glucose sensor performance.
Main Methods:
- Subcutaneous implantation of PDs with varying chamber bottom coverings (titanium mesh, cellulose acetate membrane, uncovered) in New Zealand white rabbits.
- Administration of octreotide and glucagon to induce glycemic changes.
- Histological evaluation of tissue reaction to implants.
- Analysis of subcutaneous tissue fluid glucose concentrations in relation to blood glucose levels (glycaemia).
Main Results:
- Membrane-covered PDs showed no dynamic relationship between glycaemia and subcutaneous tissue fluid glucose.
- Histology revealed seroma formation and membrane obstruction in covered devices, preventing glucose adjustment.
- Uncovered PDs demonstrated that subcutaneous tissue fluid glucose accurately reflected changes in glycaemia.
- Percutaneous devices are capable of measuring subcutaneous glucose concentration changes.
Conclusions:
- Percutaneous devices can successfully measure subcutaneous glucose kinetics.
- Porous membranes impede accurate glucose measurement in subcutaneous tissue fluid.
- Uncovered PDs serve as a viable model for studying in vivo performance of implantable glucose sensors.
- Avoidance of porous membranes is recommended for percutaneous glucose monitoring devices.