A microstructural examination of apatite induced by Bioglass in vitro

Bioglass, a clinically used bone graft material, has been tested in vitro in a simulated body fluid (SBF) up to four weeks. Apatite crystals were not only found to form on its surface but also in the reaction solutions. The apatite crystals have been examined by high-resolution transmission electron microscopy (TEM). The crystals formed in the solutions appear identical in morphology and structure with those formed on the Bioglass surface. It may be that the soluble Si in the solution serves as the nucleating site for the apatite crystal or that apatite nuclei are released from the Bioglass surface to the solution resulting in crystal growth.