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Estimation of the cellular antioxidant response to chromium action using ESR method
Tamar Kartvelishvili1, Marina Abuladze, Nino Asatiani
1Institute of Molecular Biology and Biophysics, Georgian Academy of Sciences, 14, Gotua Str., 0160 Tbilisi, Georgia. tkartvelishvili@iphac.ge
Abstract:
In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 microM (nontoxic) and 20 microM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.