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Related Experiment Videos

Antigen-specific suppressor cells in experimental autoimmune uveitis.

N A Rao1, L Atalla, S L Fong

  • 1Doheny Eye Institute, University of Southern California, Los Angeles 90033.

Ophthalmic Research
|January 1, 1992
PubMed
Summary

Anti-I-A antibodies suppress experimental autoimmune uveitis (EAU). Adoptive transfer of lymphocytes from treated rats also suppressed EAU, suggesting antigen-specific suppressor cells mediate this immunotherapy.

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Area of Science:

  • Immunology
  • Ophthalmology

Background:

  • Experimental autoimmune uveitis (EAU) is an inflammatory eye disease.
  • Anti-I-A antibodies can prevent EAU development, but the mechanism is unclear.

Purpose of the Study:

  • To investigate the mechanism of EAU suppression by anti-I-A antibodies.
  • To determine if antigen-specific suppressor cells mediate this effect.

Main Methods:

  • Adoptive transfer of lymphocytes from anti-I-A treated rats to syngeneic recipients.
  • Induction of EAU with S-antigen in recipient rats.
  • In vitro studies of lymphocyte responses to S-antigen.
  • Cyclophosphamide treatment of donor animals.

Main Results:

  • Adoptive transfer of spleen cells from anti-I-A treated rats significantly suppressed EAU development.

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  • Suppression was antigen-specific, as transfer of cells from rats primed with irrelevant antigen did not suppress EAU.
  • Pretreatment of donor animals with cyclophosphamide abrogated suppression.
  • In vitro studies showed that spleen cells from treated donors specifically suppressed lymphocyte proliferation to S-antigen.

    • Conclusions:

    • Antigen-specific suppressor cells are generated following anti-I-A antibody treatment.
    • These suppressor cells play a crucial role in the immunotherapy of EAU.