Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Robust simple sequence repeat markers for spruce (Picea spp.) from expressed sequence tags.

Dainis Rungis1, Yanik Bérubé, Jun Zhang

  • 1Department of Forest Sciences, University of British Columbia, Vancouver, Canada.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|September 8, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PCR-based species identification tools for wireworms (Coleoptera: Elateridae) of economic importance in Canada.

Pest management science·2026
Same author

Species specific marker genes for systemic defence and stress responses to leaf wounding and flagellin stimuli in hybrid aspen and silver birch.

PloS one·2026
Same author

Editorial-Announcement of the 2025 TPJ fellows.

The Plant journal : for cell and molecular biology·2025
Same author

Strong Association between Proanthocyanidins and Polysaccharides in the Cell Walls of Western Redcedar Bark.

Biomacromolecules·2025
Same author

Global meta-analysis shows action is needed to halt genetic diversity loss.

Nature·2025
Same author

The influence of tree genus, phylogeny, and richness on the specificity, rarity, and diversity of ectomycorrhizal fungi.

Environmental microbiology reports·2024
Same journal

Genome-wide association analysis and candidate gene identification for plant height in Shanxi local foxtail millet varieties.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Combined genome and transcriptome analysis of boll weight and lint percentage traits in Gossypium barbadense.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

The allelic variation of anthocyanidin reductase underlies anthocyanin biosynthesis and purple leaf trait in Brassica napus.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Unveiling core genomic regions shaping plant architecture, productivity, and seed quality traits in sesame (Sesamum indicum L.): insights from Meta-QTL study into breeding targets.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Watkins wheat landraces: a treasure of stripe rust resistance alleles identified using multi-model association analyses.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same journal

Selection of four mutant alleles of fatty acid desaturase genes for a stable high oleic and low linolenic acid soybean seed oil trait.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
See all related articles

Developing expressed sequence tag (EST) simple sequence repeat (SSR) markers from spruce ESTs provides robust genetic markers. These EST-SSR markers are valuable for comparative genomics and gene-associated studies in conifers.

Area of Science:

  • Genomics
  • Molecular Biology
  • Forest Genetics

Background:

  • Developing simple sequence repeat (SSR) markers from conifer genomic DNA is challenging due to large, repetitive genomes.
  • Expressed sequence tags (ESTs) offer an alternative approach by utilizing conserved, low-copy sequence motifs from messenger RNA.

Purpose of the Study:

  • To identify and characterize novel EST-derived SSR markers in spruce.
  • To compare the performance of EST-SSR markers with traditional genomic-derived SSR markers.

Main Methods:

  • Bioinformatic analysis of a 20,275-unigene spruce EST set to identify candidate SSRs.
  • PCR amplification and polymorphism screening of candidate EST-SSR markers across white, Sitka, and black spruce.
  • Testing previously described genomic-derived spruce SSRs for cross-species amplification.

Related Experiment Videos

Main Results:

  • Identified 44 candidate EST-SSR markers, with 25 amplifying and showing polymorphism in three spruce species.
  • 101 previously described genomic-derived SSRs were tested, with 17 amplifying across the three species.
  • EST-SSRs exhibited lower heterozygosity and apparent inbreeding compared to genomic-derived SSRs, suggesting fewer null alleles.

Conclusions:

  • EST-SSR markers are robust and suitable for comparative studies in spruce.
  • EST-SSRs are located within expressed regions, enhancing their utility for gene-associated quantitative trait loci mapping and detecting selection at specific genes.