Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differences in snRNP localization between transformed and nontransformed cells.

D L Spector1, G Lark, S Huang

  • 1Cold Spring Harbor Laboratory, New York 11724.

Molecular Biology of the Cell
|May 1, 1992
PubMed
Summary

The organization of small nuclear ribonucleoproteins (snRNPs) differs in mammalian cells based on their physiological state. Transformed and immortal cells show distinct coiled body staining patterns compared to normal cells, indicating changes upon transformation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatic instability of carotenoid biosynthesis in the tomato ghost mutant and its effect on plastid development.

Planta·2013
Same author

Role of H3K27 demethylases Jmjd3 and UTX in transcriptional regulation.

Cold Spring Harbor symposia on quantitative biology·2011
Same author

Methylation of histone H3 at Lys-9 is an early mark on the X chromosome during X inactivation.

Cell·2001
Same author

Nuclear domains.

Journal of cell science·2001
Same author

Visualization of gene activity in living cells.

Nature cell biology·2001
Same author

Compartmentalization of RNA processing factors within nuclear speckles.

Journal of structural biology·2000

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Small nuclear ribonucleoproteins (snRNPs) are crucial for pre-mRNA splicing.
  • The localization and organization of snRNPs within the nucleus can reflect cellular state.
  • Coiled bodies are nuclear organelles implicated in snRNP metabolism.

Purpose of the Study:

  • To investigate the nuclear localization patterns of snRNPs in mammalian cells.
  • To determine if snRNP organization differs between normal, immortalized, and transformed cells.
  • To correlate snRNP localization with cellular physiology, including transformation and immortalization.

Main Methods:

  • Immunolabeling of mammalian cells using antibodies against specific snRNP components (anti-Sm, anti-B'', anti-m3G).

Related Experiment Videos

  • Microscopic examination to observe snRNP localization patterns, focusing on speckled nuclear staining and coiled body labeling.
  • Quantification of cells exhibiting specific staining patterns in different cell types (defined passage, immortal, transformed).

    • Main Results:

    • Cells of defined passage number showed a speckled snRNP staining pattern with minimal coiled body labeling (2-3%).
    • Transformed cells displayed intense coiled body labeling in 81-99% of cells, alongside speckled patterns.
    • Immortal cells exhibited intermediate coiled body labeling (4-40%) with distinct staining characteristics.
    • Adenovirus-transformed immortal cells showed a significant increase in intense coiled body labeling (24% to 99%).

    Conclusions:

    • The nuclear organization of snRNPs serves as an indicator of cellular physiology.
    • Cellular transformation and immortalization lead to discernible alterations in snRNP localization, particularly affecting coiled bodies.
    • SnRNP organization provides insights into the molecular changes associated with neoplastic progression and cellular aging.