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Identification of recoverin-like immunoreactivity in mouse brain
1Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Brain Research
|February 7, 1992
Abstract:
Mouse brain was found by immunoblot analysis to have a protein of Mr 23,000 (P23k) that was clearly different from recoverin and was labeled with an antiserum raised against the NH2-terminus of recoverin. P23k could not be detected by an antiserum raised against the COOH-terminus of recoverin. Blots with 45Ca demonstrated that P23k bound Ca2+ and had a Ca(2+)-dependent membrane-binding property. Immunohistochemical studies provided the specific distribution of P23k in cerebral cortex and cerebellum.