Related Experiment Video
Updated: Aug 22, 2026

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol
Published on: April 2, 2018
Chiral separation of omeprazole and several related benzimidazoles using supercritical fluid chromatography
M Jesús del Nozal1, Laura Toribio, José L Bernal
1Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, C/Prado de la Magdalena s/n, 4700-Valladolid, Spain.
Abstract:
A study of the enantiomeric separation of omeprazole and several related benzimidazoles, using supercritical fluid chromatography (SFC), on the amylose based column Chiralpak AD is presented in this work. The effect of the organic modifier as well as temperature on the retention and enantioresolution was investigated. Alcohol-type modifiers provided the best results, allowing the enantiomeric separation of all the compounds studied with resolutions that were in most cases higher than 2, and analysis times lower than 10 minutes. An investigation of the temperature effect revealed that the isoelution temperature was below the working temperature range in only two cases, and hence it was better to work at the highest temperature permitted.
Related Concept Videos
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Elution Process
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

