Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved segmentation method for in vivo microCT imaging.

Jan H Waarsing1, Judd S Day, Harrie Weinans

  • 1Department of Orthopaedics, Erasmus Medical Centre, Rotterdam, The Netherlands.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|September 10, 2004
PubMed
Summary

A new local thresholding method accurately segments bone in microCT scans, especially in vivo where scan quality is limited. This automated approach improves bone morphometry analysis without increasing radiation exposure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linking genetic variants to bone microstructure: Histological signatures of osteogenesis imperfecta subtypes.

Bone·2026
Same author

Fracture risk assessment by dual-energy absorptiometry in osteogenesis imperfecta: A systematic review of measurement sites and its predictive value.

Bone reports·2026
Same author

Impact of quantified knee positioning on the measurement of minimal joint space width using statistical shape models: A cross-sectional and longitudinal analysis in the IMI-APPROACH.

Osteoarthritis imaging·2026
Same author

Tibial genu varum and primary cam morphology in healthy young adults: A cross-sectional study uncovering the double threat to joint health.

Osteoarthritis and cartilage open·2026
Same author

Harnessing copper-strontium synergy in an injectable composite for multi-pathway bone regeneration.

RSC advances·2026
Same author

The Effects of Radioimmunotherapy and Antibiotics on Biofilm-Associated Implant Infections in a Preclinical Rat Model.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026

Area of Science:

  • Biomedical Engineering
  • Radiology
  • Materials Science

Background:

  • Accurate bone segmentation in microCT is crucial for reliable bone morphometry.
  • Challenges include noise, resolution limits, and beam-hardening, particularly in low-dose in vivo microCT.
  • Standard global thresholding methods can introduce bias in architectural measurements.

Purpose of the Study:

  • Introduce and validate a novel automated local thresholding method for microCT image segmentation.
  • Compare its performance against standard global thresholding techniques.
  • Assess the method's robustness under varying scan quality conditions.

Main Methods:

  • Developed and applied a local thresholding segmentation algorithm.
  • Validated segmentation accuracy against histology.

Related Experiment Videos

  • Investigated sensitivity to signal-to-noise ratio and resolution compared to global methods.

    • Main Results:

    • The local threshold method achieved high accuracy, comparable to histology, especially on high-quality scans.
    • It demonstrated robustness to degraded scan quality, with minor performance impact.
    • Global methods struggled to segment heterogeneous bone structures (cortex and trabeculae) with a single threshold.

    Conclusions:

    • The local threshold method significantly outperforms global methods for segmenting heterogeneous bone structures at lower resolutions.
    • It enhances the utility of in vivo microCT by providing accurate bone morphometry without extended scan times or increased radiation.
    • This method maximizes the potential of microCT for preclinical and clinical bone analysis.