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Updated: Aug 22, 2026
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
Published on: May 31, 2020
[The expression of CXCR4 on acute leukemia cells and its implication for extramedullary infiltration]
Sheng Li1, Zi-xing Chen, Wei Wang
1The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Objective:
To study the expression of CXCR4 in acute leukemic cells and its clinical significance.
Method:
Bone marrow samples from 73 acute leukemia patients and leukemic cell lines were investigated by flow cytometry (FCM), the expression of SDF-1 in human marrow stromal cells and meninges were studied by using reverse transcription polymerase chain reaction (RT-PCR). Adhesion, migration and invasion of U937, NB4 and K562 cells were studied in vitro.
Results:
The expression rates of CXCR4 in ALL and AML patients was 65.6% and 17.1%, respectively. And it was 0.2%, 41.0% and 52.0% in K562, U937 and NB4 cells, respectively. The extramedullary infiltration rates were 61.9% and 18.2% for CXCR4 positive and negative groups of ALL, respectively (P < 0.05); while in AML, the number of peripheral white blood cells in CXCR4 positive group was lower than that in CXCR4 negative group (P < 0.05). SDF-1alpha could enhance the adhesion, migration and invasion capacity of leukemic cells in vitro.
Conclusion:
Overexpression of CXCR4 in AL cells might be the molecular mechanism of extramedullary infiltration in leukemia.
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