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Related Experiment Videos

The small subunit processome is required for cell cycle progression at G1.

Kara A Bernstein1, Susan J Baserga

  • 1Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA.

Molecular Biology of the Cell
|September 10, 2004
PubMed
Summary

Yeast cells require sufficient ribosome biogenesis for growth. Depletion of small subunit processome proteins causes cell cycle arrest in G1, indicating a critical size must be reached before division.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Ribosome biogenesis is essential for protein translation and cellular growth.
  • Cell cycle regulation ensures proper cell division and organismal development.
  • Metazoan cells exhibit cell cycle-dependent regulation of ribosome biogenesis.

Purpose of the Study:

  • To investigate whether ribosome biogenesis is cell cycle regulated in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
  • To identify cellular sensors linking cell size and ribosome biogenesis.
  • To understand the role of the small subunit (SSU) processome in cell cycle progression.

Main Methods:

  • Comparative analysis of ribosome biogenesis regulation in yeast versus metazoan cells.
  • Investigation of the SSU processome, including Nan1/Utp17, and its mitotic regulation.

Related Experiment Videos

  • Cell cycle analysis (G1, G2, M phases) upon depletion of SSU processome proteins.

    • Main Results:

    • Ribosome biogenesis is not regulated similarly in yeast compared to metazoan cells.
    • The SSU processome and Nan1/Utp17 are not mitotically regulated.
    • Depletion of SSU processome proteins leads to G1 cell cycle arrest, independent of Nan1/Utp17 function.
    • Yeast cells must reach a critical size, dependent on ribosome number, to progress through G1 and divide.

    Conclusions:

    • Yeast ribosome biogenesis is not directly tied to mitotic regulation in the same way as in metazoans.
    • Cellular growth to a critical size, supported by sufficient ribosome production during G1, is necessary for yeast cell division.
    • The SSU processome plays a crucial role in חייב cell cycle progression by ensuring adequate ribosome biogenesis for growth.